Composite

Part:BBa_K2570018:Design

Designed by: Luying He   Group: iGEM18_FJNU-China   (2018-10-01)


Production of PLA: TyrB+LDH+rocG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2054
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2839
    Illegal NgoMIV site found at 3378
    Illegal AgeI site found at 1115
    Illegal AgeI site found at 2527
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We did site-directed mutation for hns in order to follow the part-submitted rules.


Source

D-lactate dehydrogenase Dldh from Lactobacillus bulgaricus ATCC 11842

Glutamate dehydrogenase rocG from Bacillus subtilis

Phenylalanine aminotransferase Tyrb from Escherichia coli 21B.

References